RO should be fine. i actually use tap water to hatch mine, as my city water does not use clorine. i only get about a 30-40% hatch rate from it because of the lower osmotic pressure, but when we are talking ten thousand eggs for the first generation or so, in five gallons of water, it doesnt really matter. every time i dry out my sand in my five gallon(dont use the kiddie pool anymore), i end up with far more hatches than i could ever sustain anyway.
there is a live marine algae supplement for reef tanks called phyto feast that i use to establish green water. i discovered it a while ago while experimenting with it. it seems that the "marine" algae they use can also live in fresh water.
one thing i forgot to mention is that you will have better luck if you season your tank/pool first. to do this, simply fill it about half way with water, add clean sand to the bottom, oak/hardwood leaves, and any source of green water. simply leave it out in the sun until it dries. after it is dry, you will be ready to add the eggs and water to start raising your fairy shrimp.
seasoning the tank first puts it as close to the natural vernal pool habitat as you can get. it allows algae time to bloom, go through a cycle, and then produce spores that will begin growing imediately upon rehydration. this works much better than simply using oak leaves alone, although i still find that there is less die off due to bacteria and fungus when the tanins from oak leaves are present. it basicaly just gives the new nauplii a food source that they can eat to help them through the first week, but not so much food that it fouls the water.
the type of leaves is not all that important, even pine needles will work in a pinch, but they MUST be seasoned first. any kind of hardwood leaf should be fine. the idea is that they almost imediately release tannins into the water, which inhibit fungus, while at the same time breaking down and producing bacteria for the nauplii to eat. about 12 years ago i first came up with the idea using half rotten oak bark. i couldnt for the life of me get Triops longicaudatus to surive their first week, but noticed that if i added a tea bag to their water on the first day, they lived longer. that is the idea that led me to the oak bark, as it seemed to produce a more stringent tannin. oak bark led to leaves, as i realized it was easier to grind up and just as effective.
Gerald, where did you find fairy shrimp in the game lands? i have been out there around this time for the past few years and have never seen them. i have been raising the species streptocephalus seali for a few years now and would love to see them in the wild. maybe im hitting them up too early?
my current culture was started right before i came home from iraq this past december and i still have one large green male that is still looking good after 121 days. my wife wanted to surprise me with an active fairy shrimp tank.
Edited by Auban, 26 March 2012 - 08:42 PM.