A graduate student in my lab, Rich Harrington, recently published a paper that examines the evolutionary history of egg mimicry in spottail darters, a clade we named Stigmacerca in our 2011 darter phylogeny paper.
Rich's work is based on an analysis of DNA sequences from 15 nuclear genes and a single mtDNA gene. The analyses show that there is extensive mtDNA introgression, where mtDNA genomes are crossing species boundaries because of hybridization. Also, there is gene flow between species detected in the nuclear genes. In particular, there is gene flow between Etheostoma nigripinne and E. neopterum.
Another important discovery in this paper is that the Clarks River population of Etheostoma oophylax is more closely related to the federally protected species E. chienense, than it is to other populations of E. oophylax.