This is pretty much the setup they're currently in.
Tank Size: 20 High (24"x12"x16")
Substrate: Off-white Pea Gravel
Decorations: A bunch of smaller rocks with a few larger ones and some hornwort
Filtration: Sponge Filter
WCs: 50% weekly
Temp: Currently 68F (once I have my Fishroom setup in Jan and move the tank down there it'll probably get down into the low 50s in the winter.
pH: 7.6
Tankmates: None at the moment, I may be getting a school of flame chubs in dec that I'd house with them temporarily but that'd only be until I could setup a 20L for the chubs about a month later so it wouldn't interfere with breeding activity.
The only problem is that I think I have a bad sex ratio. I'm not certain I sexed them right but if the rule applies that females are smaller and less vividly colored than males, then I have 4 males and 1 female unfortunately. One of the males (however not the biggest strangely) is very dominant over the others and will often bully all the others, the female's dorsal fins are fairly torn up sadly and the other males don't get treated to well either, however nobody is injured to the point of injury or isolating themself. Though they are in my experience the most skittish darters I own or have owned and I have to be perfectly still when watching them lest they run and hide.
My plan is once I have my Fishroom setup that I'll move the tank down there and only have the female and either one or two males in the setup. I'd let the temp gradually drop to around 50-52 (lower if I can get in any farther down) and take the direct light down to roughly 6 hours a day. Feedings would only be once a day or once every other day at this point with frozen being their main diet. Come spring I'd raise the temperature up to 60-64 and the light up to about 10-12 hours a day and hope for spawning action. I'd feed them twice a day live foods now. The tank would be a thin layer of sand for the substrate in spread of gravel and have multiple rocks arranged in caves for spawning, since Johnnys are "upside down Spawners" there'd be plenty of room on the underside of the rocks suitable for laying eggs. If I see any eggs I'd remove the parents and leave the fry to hatch at which point I'd feed them very small foods until they grew large enough for frozen foods. That's pretty much what I plan on doing.
Does anyone see any problems in this or anything I should change for a better chance of spawning?