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Stripping Fathead Minnows


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#1 ColbyJohnson

ColbyJohnson
  • NANFA Guest
  • Minnesota

Posted 30 August 2022 - 03:28 PM

Hi all,

I am a graduate researcher at the University of Minnesota whose project would be greatly benefited by having access to 1-cell stage fathead minnow embryos. I was thinking in vitro fertilization would be the easiest route to them, but I have had trouble getting milt from male fatheads. I am wondering if anyone here has stripped fatheads or knows of someone who has that I could talk to about their protocol?



#2 centrarchid

centrarchid
  • NANFA Guest

Posted 01 September 2022 - 02:35 PM

Fish I have worked with that are not egg scatterers tend not to produce copious volumes of milt. Therefore, with species like Fathead Minnows, I would sacrifice male, remove and mince gonads and before diluting and using milt to fertilize eggs.


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#3 ColbyJohnson

ColbyJohnson
  • NANFA Guest
  • Minnesota

Posted 07 September 2022 - 11:55 AM

Fish I have worked with that are not egg scatterers tend not to produce copious volumes of milt. Therefore, with species like Fathead Minnows, I would sacrifice male, remove and mince gonads and before diluting and using milt to fertilize eggs.

Good insight, thank you!



#4 mamarich4

mamarich4
  • NANFA Guest
  • NYC USA

Posted Yesterday, 05:09 AM

interesting information


Edited by mamarich4, Yesterday, 05:10 AM.




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