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Sterilizing a culture vat


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#1 Moontanman

Moontanman
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Posted 24 May 2015 - 03:59 PM

How much clorox would it take to sterilize a 360 gal fiberglass tank? I suspect that I have hyra or some other pest that is keep my daphnia from colonizing the tank. The tank has rough walls, not smooth. 


Michael

Life is the poetry of the universe
Love is the poetry of life

#2 gzeiger

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Posted 25 May 2015 - 11:49 AM

Not sure on the dose, but would it be impractical to just drain and let dry a couple days?



#3 Moontanman

Moontanman
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Posted 25 May 2015 - 05:41 PM

Not sure on the dose, but would it be impractical to just drain and let dry a couple days?

 

 

I thought Hydra have a resting phase to survive drying out? 


Michael

Life is the poetry of the universe
Love is the poetry of life

#4 smilingfrog

smilingfrog
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Posted 25 May 2015 - 11:22 PM

I have usually read that a 1:10 solution of bleach in water will kill most things. The times I've bleached a tank or bucket, I've figured bleach is cheap and bumped it up a little just to be sure. If I were doing something that large though, I think I would drain it, put straight bleach into a spray bottle, and then spray all surfaces making sure they were good and wet.

#5 littlen

littlen
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Posted 26 May 2015 - 08:51 AM

My thoughts are if you're trying to kill everything (hydra) is there really such a thing as "too much" bleach?  Go crazy and add a whole jug and let it run through the system for a few days.  Then drain, refill, and let it run with some thio to neutralize any residual chlorine.  

A thorough drying afterwards couldn't hurt either.


Nick L.




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