Hi all,

I am a graduate researcher at the University of Minnesota whose project would be greatly benefited by having access to 1-cell stage fathead minnow embryos. I was thinking in vitro fertilization would be the easiest route to them, but I have had trouble getting milt from male fatheads. I am wondering if anyone here has stripped fatheads or knows of someone who has that I could talk to about their protocol?

Fish I have worked with that are not egg scatterers tend not to produce copious volumes of milt. Therefore, with species like Fathead Minnows, I would sacrifice male, remove and mince gonads and before diluting and using milt to fertilize eggs.

Fish I have worked with that are not egg scatterers tend not to produce copious volumes of milt. Therefore, with species like Fathead Minnows, I would sacrifice male, remove and mince gonads and before diluting and using milt to fertilize eggs.

Good insight, thank you!

interesting information

Edited by mamarich4, 23 September 2022 - 05:10 AM.