pirate perch culture
Posted 26 March 2010 - 10:53 AM
[quote name='centrarchid' date='23 March 2010 - 12:21 PM' timestamp='1269361277' post='74251']
They are not fertile (no male present) despite some going through some early cell divsions. [quote]
Posted 26 March 2010 - 12:24 PM
Water exposure of eggs alone can often initiate egg activation but no hatch likley since quality of activation may be inadequate to promote hatch. Other factors can stimulate activation better resulting in hatch without involvement of paternal DNA. I use latter in my work to create gynogens. Controls (negative controls) used for the process are not exposed to sperm but cell division does commence in most eggs although hatch does not.
Posted 27 March 2010 - 09:02 AM
Bad news first.
Only about 50% of eggs I collected from a brood produced two days ago contain developing embryos. Just 13 are alive with hearts beating.
Secondly, my water flea culture (Daphnia magna) appears to be in the process of crashing. Centrarchid pointed out the "ephippia" in the image and the black patches on most of my adult water fleas. He says they are no longer reproducing parthenogenically, which is needed for sustainable harvest. He says start new cultures ASAP.
Good news is I have a lot more than 13 developing embryos. See photograph below. When looking at image, you could see hearts of the pirate perch embryos beating. Egg diameter of this clutch averages just under 1.8 mm. To the lower right of the pirate perch is one of the "ephippiums". Apparently when the pirate perch are fighting, scales are nocked off from time to time. The males do spend a lot of time bitting other fish.
PIRATE PERCH EMBRYOS 1.0x 2010 March 27 0800 001.jpg 265.3KB 4 downloads
Posted 27 March 2010 - 02:58 PM
Posted 27 March 2010 - 07:51 PM
IMG_0434.JPG 34.72KB 1 downloads
Other males hide within the nest site and frequently flutter their pectoral fins, often for several seconds at a time. They also will come out to challenge other fish that approach especially other pirate perch. Notice the males yellow under parts; all breeding males seem so colored.
IMG_0439.JPG 36.75KB 1 downloads
These picture shows how the females just sit tight and capture what ever edible comes by. In this case water fleas.
IMG_0435.JPG 29KB 1 downloads
IMG_0436.JPG 39.58KB 1 downloads
The last image shows tank being used to incubate pirate perch yet to hatch. Parents were in it until yesterday. Hopefully all tanks will go thru this sequence.
Posted 28 March 2010 - 11:41 AM
Posted 28 March 2010 - 03:28 PM
Posted 29 March 2010 - 08:07 AM
Posted 29 March 2010 - 08:13 PM
PIRATE PERCH PROLARVAE 1.25x 2010 March 29 2000 001.jpg 187.31KB 2 downloads
Posted 31 March 2010 - 06:54 AM
PIRATE PERCH PROLARVAE 1.25x 2010 March 30 2000 001.jpg 196.86KB 1 downloads
Posted 31 March 2010 - 03:00 PM
PIRATE PERCH PROLARVAE 1.25x 2010 March 31 2000 001.jpg 185.74KB 1 downloads
The second picture the blood can even be seen to flow through capillaries, not just heart and arteries. The faint speckling on tail area I think is individual blood cells. They are also assymetrical in respect to position of yolk.
PIRATE PERCH PROLARVAE 2.0x 2010 March 31 2000 001.jpg 132.38KB 1 downloads
I wonder how dad keeps from eating these guys. They move around a lot. He does not seem to eat water fleas very well.
Posted 31 March 2010 - 03:34 PM
Watch and see if the male spits out any fry he slurps up, like Elassoma do.
Posted 31 March 2010 - 03:41 PM
Posted 31 March 2010 - 05:16 PM
Posted 31 March 2010 - 08:06 PM
Your magnification isn't high enough to pick up individual blood cells in the tail in that last image. I'd guess that the speckling you mention (a series of grayish spheres often in loose linear order) is cartilage forming to define the caudal rays. Others probably know better than me.
The movement of the "blood cells" was very obvious as particles following very specifc routes. The irregular rows are made up of the particles I am seeing. Their movement also appeared to be in time with heart beats. I would like to make a short movie of the action. If I am correct then we are likely to conclude the blood cells in such young pirate perch are very large. Also the blood completes a circuit between heart and body within a second or so.
Edited by Pirateperch, 31 March 2010 - 08:10 PM.
Posted 31 March 2010 - 09:19 PM
Posted 31 March 2010 - 09:31 PM
Lense of eye piece is 10x and larger image was made with 2.0x "objective" lense. I do not know what magnification of digital part is.
The larvae I think are just under 6 mm long. I can measure them tommorow to make certain. The scope can measure if correct magnification is known. Supposedly the microscope I am using can make video clips as well.
Posted 31 March 2010 - 10:05 PM
Posted 01 April 2010 - 03:08 AM
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