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pirate perch culture


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#41 Guest_Pirateperch_*

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Posted 01 April 2010 - 08:39 AM

All I have to say is WOW that is really cool and thanks for the updates!


Thanks Airbrn1187, I will try to keep them coming.


Fundulus,

The microscope I am using is driven by Image Pro Express.

Lengths at age relative to hatching;

0 hours
24 hours 5.0 mm
48 hours 5.8 mm
60 hours 5.6 mm
72 hours will be this afternoon




I took ten photos in one second to make a video clip. They are too large to upload as is. I will try to reduce resolution. They do show movement and excellent heartbeats!


Ed

#42 Guest_fundulus_*

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Posted 01 April 2010 - 12:35 PM

I measured one of the larvae in the images above with the objects pulsing through it. The larva is 5.5 mm TL. I stand corrected, I'd say that the objects seen pulsing in rhythm to the heart are probably blood cells. They're about 10 micrometers in diameter, what you'd expect for red blood cell size. Under the right light I guess that circulating blood cells can be seen at 20X.

#43 Guest_Pirateperch_*

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Posted 01 April 2010 - 01:59 PM

Updated image of development.

Age after hatching relative to length.
0 hours 3.5 mm
24 hours 5.0 mm
48 hours 5.8 mm
60 hours 5.6 mm
72 hours 5.5 mm


The following image is of a pirate perch at 72 hour after hatching. They are starting to be able to right themselves. Next I will likely have to kill them before I take pictures. Pectoral fins are still not visible.

Attached File  PIRATE PERCH PROLARVAE 1.25x 2010 April 1 2010 001.jpg   149.75KB   0 downloads


The reduced length may be real as animals age and restructure tissues.

#44 Guest_Pirateperch_*

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Posted 02 April 2010 - 02:29 PM

Pirate perch are now about 96 hours old from hatch. They are getting noticeably darker such that they appear to have a strip going down their back. Think they are also starting to breath through their gills. They are about 5.5 mm long, maybe a tad less. Still shrinking as head and tail change shape.

Attached File  PIRATE PERCH PROLARVAE 1.25x 2010 April 2 2010 001.jpg   172.96KB   0 downloads

The second image has more light coming from top. The eyes look functional now.

Attached File  PIRATE PERCH PROLARVAE 1.25x 2010 April 2 TOP LIGHT 001.jpg   200.72KB   0 downloads

#45 Guest_centrarchid_*

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Posted 03 April 2010 - 11:17 AM

In respect to observations regarding parents and young, I suspect the natural interactions will have to wait until next generation. Otherwise Pirateperch, you will not have a many young as you want and still have enough young to bring in for photographs. You will still have to wait a year to see what you want to see.

Edited by centrarchid, 03 April 2010 - 11:53 AM.


#46 Guest_Pirateperch_*

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Posted 03 April 2010 - 04:48 PM

Pirate perch at 120 hours after hatch. The length is now about 5.9 mm. The anus is now evident, where it should be for most fish species.

They seem to have left the poly floss and swim some distance when disturbed. Seems like they would be easy pickings for visually oriented predators on larval fishes.

Attached File  PIRATE PERCH PROLARVAE 1.25x 2010 April 3 001.jpg   210.85KB   0 downloads

#47 Guest_fundulus_*

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Posted 03 April 2010 - 04:52 PM

Yeah, with most of the yolk sac absorbed they should be closer to neutral buoyancy. And they're certainly easy pickings for almost anything bigger than they are that finds them.!

#48 Guest_Pirateperch_*

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Posted 03 April 2010 - 09:22 PM

They are getting closer to being neutrally bouyant but still no swim bladder. Nor can I see pectoral fins. They seem to get around like freshly hatched salamanders which also fall gracefully onto the bottom and just sit their.

#49 Guest_Pirateperch_*

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Posted 04 April 2010 - 08:11 AM

As of this morning the pirate perch are now 132 hours old. I can see pectoral fins but they do not move, at least not at the hummingbird pace exhibited by so many other fishes of the same age or size.

#50 Guest_Pirateperch_*

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Posted 04 April 2010 - 05:22 PM

Pirate perch now 144 hours after hatching. The areas that will become dorsal and and anal fins now stand out relative to rest tissue that has been srving as single median fin. Pectoral fins now move. Tail looks like that of an adult spring cave fish tail. Tail fin of lower fish looks cloudy and yolk not shrinking like it should. Very likely that fish will die do to handling or poor water quality. I think they will be eating in about two days.

Attached File  PIRATE PERCH PROLARVAE 1.25x 2010 April 4 001.jpg   192.28KB   0 downloads

#51 Guest_Pirateperch_*

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Posted 05 April 2010 - 03:32 PM

The pirate perch are now dorsal side up all the time. Had to kill them to photograph from side.


Top view, lots of pigment. See the pectoral fins.

Attached File  PIRATE PERCH PROLARVAE 1.25x 2010 April 5 Dorsal Aspect 001.jpg   200.52KB   0 downloads

The money shot seems to indicate deformities developing in animals held in small tupperware container. Form this point only only fish reared in aquarium since hatch will be sampled.

Attached File  PIRATE PERCH PROLARVAE 1.25x 2010 April 5 001.jpg   198.85KB   0 downloads

#52 Guest_Pirateperch_*

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Posted 06 April 2010 - 04:36 PM

Pirate perch 192 hour after hatch are at cusps of becoming larvae. They are swimming about now, dispersing. They may slao be taking first exogenous food but yolk still present. Length 6.7 mm.

Attached File  PIRATE PERCH PROLARVAE 1.25x 2010 April 6 001.jpg   200.63KB   0 downloads

#53 Guest_Pirateperch_*

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Posted 06 April 2010 - 08:02 PM

They eat baby brine shrimp but I am pretty sure they are not using their eyes. Brine shrimp behind the larval pirate perches head are at just as much risk as those in front. I thought surely they would go through a stage where the eyes were used.

#54 Guest_BTDarters_*

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Posted 06 April 2010 - 10:42 PM

Nice pics and updates! Thanks for sharing! If you want, you could turn the pictures and some text into an article for the AC. What do you think?

Brian

#55 Guest_Pirateperch_*

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Posted 07 April 2010 - 07:03 AM

Nice pics and updates! Thanks for sharing! If you want, you could turn the pictures and some text into an article for the AC. What do you think?

Brian


It appears pirate perch have been beat to death with articles in the American Currents. Little room left to present new information in respect to life history observations. What I can do is generate a cook book like proceedure for getting off good spawns using standardizable methods. It could start with acquiring brood stock and end with first feeding larvae. Afterwards,I think very few folks here would be challenged with rearing these animals.

Edited by Pirateperch, 07 April 2010 - 07:38 AM.


#56 Guest_BTDarters_*

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Posted 12 April 2010 - 12:29 AM

It appears pirate perch have been beat to death with articles in the American Currents. Little room left to present new information in respect to life history observations. What I can do is generate a cook book like proceedure for getting off good spawns using standardizable methods. It could start with acquiring brood stock and end with first feeding larvae. Afterwards,I think very few folks here would be challenged with rearing these animals.


Sounds good. I'd like to see that!

Brian

#57 Guest_Pirateperch_*

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Posted 12 April 2010 - 09:18 PM

Sounds good. I'd like to see that!

Brian


Brian,

I am well into writing this up. Where should it be submitted? What format?


Ed

#58 Guest_BTDarters_*

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Posted 13 April 2010 - 04:20 AM

Ed,

You can submit the paper to me in PDF, older MS Word (not .docx), OpenOffice.org or text format. If the file size is small enough, you can email it to me at bt@btdarters.com. I can take anything up to about 10 MB. If you need to snail-mail me a CD, please mail it to:

Brian J. Torreano
1414 W. Sunset Rd., #102
Port Washington, WI 53074

I'm looking forward to your submission!

Brian

P.S. Please send any photos in the highest resolution possible. Thanks!

Edited by BTDarters, 13 April 2010 - 04:21 AM.


#59 Guest_Pirateperch_*

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Posted 11 May 2010 - 04:01 PM

Brian,

How do I have images for this article? Images within it or as separate documents? I want to make certain images are in correct sequence in respect to text.

Ed

#60 Guest_BTDarters_*

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Posted 11 May 2010 - 06:49 PM

Ed,

If you could send the images separately from the text, that would be best. You can reference the images in the text by doing something like this:

AC-Catch.jpg
Precariously attempting to net a female Sturgeon from a spawning group.

AC-Ed&CarlosCatch.jpg
Ed & Carlos hastily carrying a freshly netted male Sturgeon to the work station.

AC-Jackie&Equipment.jpg
Jackie preparing her egg and milt collection equipment for the day.

AC-MeasuringBoard.jpg
The Wisconsin DNR Crew bringing a newly caught Lake Sturgeon to the measuring board.

AC-MiltCollection.jpg
Jackie collecting milt with a syringe from a male Sturgeon.


Then, when you've got the images and text ready to go, zip them all up in a zip file and email them along to me. My email address is below. Again if the final file size is over 10 MB, we'll have to have you snail-mail them to me on a CD or something. Just let me know. If you have any other questions, just let me know. Thanks!

Brian




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